Device

Part:BBa_I715033

Designed by: Amanda Valencia   Group: iGEM07_Davidson_Missouri_W   (2007-07-18)


lacIq reverse-RBS reverse-pLac-RBS-HinLVA-TT

This device generates Hin recombinase that can flip DNA between hixC sites. The Hin cds is under the control of the lac promoter and also has a degradation tag. Expression to the left of the lacIq repressor is intended to prevent Hin expression in the absence of inducer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None